Single-stranded microRNA mimics
نویسندگان
چکیده
منابع مشابه
Circular Single-Stranded Synthetic DNA Delivery Vectors for MicroRNA
Single-stranded (ss) circular oligodeoxynucleotides were previously found to undergo rolling circle transcription (RCT) by phage and bacterial RNA polymerases (RNAPs) into tandemly repetitive RNA multimers. Here, we redesign them to encode minimal primary miRNA mimics, with the long term aim of intracellular transcription followed by RNA processing and maturation via endogenous pathways. We des...
متن کاملArgonaute 2-dependent Regulation of Gene Expression by Single-stranded miRNA Mimics
MicroRNAs (miRNAs) are small noncoding transcripts that regulate gene expression. Aberrant expression of miRNAs can affect development of cancer and other diseases. Synthetic miRNA mimics can modulate gene expression and offer an approach to therapy. Inside cells, mature miRNAs are produced as double-stranded RNAs and miRNA mimics typically retain both strands. This need for two strands has the...
متن کاملUsing DharmaconTM Mimics and Inhibitors for microRNA Functional Analysis
Introduction: microRNAs (miRNAs) are naturally occurring short RNA molecules of about 22 nucleotide (nt) long that regulate gene expression by binding to target mRNA and suppressing its translation or initiating its degradation.1 Since their initial description in C. elegans in 1993, almost four thousand miRNAs in over 40 species of animals, plants and viruses have been identified.2 Subsequent ...
متن کاملTransfection of microRNA Mimics Should Be Used with Caution
Transient transfection of chemically synthesized microRNA (miRNA) mimics is being used extensively to study the functions and mechanisms of endogenous miRNAs. However, it remains unclear whether transfected miRNAs behave similarly to endogenous miRNAs. Here we show that transient transfection of miRNA mimics into HeLa cells by a commonly used method led to the accumulation of high molecular wei...
متن کاملRNA virus microRNA that mimics a B-cell oncomiR.
MicroRNAs (miRNAs) are small RNAs that play a regulatory role in numerous and diverse eukaryotic cellular processes. Virus-encoded miRNAs have garnered much interest, although the functions of most remain to be deciphered. To date, readily detectable, evolutionarily conserved natural miRNAs have only been identified from viruses with DNA genomes. Combined with the fact that most miRNAs are gene...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: RNA
سال: 2012
ISSN: 1355-8382
DOI: 10.1261/rna.031278.111